Scott Laboratory - Immios Biologics

Scott Laboratory Website

Canadian Blood Service • University of British Columbia • Centre for Blood Research • Vancouver, BC  Canada

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Polymer-Based Bioengineering Solutions for Immune Modulation and the Prophylactic Inhibition of Viral Infections

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The 'immunocamouflage' of foreign cells and tissues via cell surface modification with biocompatible polymers such as methoxypoly(ethylene glycol) [mPEG] can be used to modulate the immune response both in vitro and in vivo.  This immune modulation arises consequent to the upregulation of regulatory T cells (Tregs) and the down regulation of effector (Teff; pro-inflammatory) T cells.  The INCREASE in the Treg:Teff ratio induces a tolerogenic state in the treated animal.

Polymer-Based Bioengineering for Immune Modulation

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Le, Y. and Scott, M.D. Immunocamouflage: The Biophysical Basis of Immunoprotection by Grafted Methoxypoly(Ethylene Glycol) [mPEG].  Acta Biomaterialia, 6:2631-2641 (2010).

Wang, D., Kyluik, D.L, Murad, K.L., Toyofuku, W.M., and Scott, M.D.  Polymer-Mediated Immunocamouflage of Red Blood Cells: Effects of Polymer Size on Antigenic and Immunogenic Recognition of Allogeneic Donor Blood Cells. Science China – Life Sciences, 54:589-598 (2011).  [NOTE:  Cover Art and featured article]

Wang, D., Toyofuku, W.M., Chen, A.M. and Scott, M.D. Induction of Immunotolerance via mPEG Grafting to Allogeneic Leukocytes.  Biomaterials 32:9494-9503 (2011).

Wang, D., Toyofuku, W.M., Scott, M.D. The Potential Utility of Methoxypoly(Ethylene Glycol)-Mediated Prevention of Rhesus Blood Group Antigen RhD Recognition in Transfusion Medicine.  Biomaterials, 33:3002-3012. (2012)

Wang, D., Toyofuku, W.M. Kyluik, D.L. and Scott, M.D. Chapter 7:  Use of Flow Cytometry In The In Vitro and In Vivo Analysis of Tolerance/Anergy Induction By Immunocamouflage.  In: Flow Cytometry-Recent Perspectives, (Editor: Schmid, I.) InTech, ISBN 979-953-307-355-1. (2012).  Pages 133-150.  Link:  Intech Web Page

Le, Y. Li, L., Wang, D. and Scott, M.D. Immunocamouflage of Latex Surfaces by Grafted Methoxypoly(Ethylene Glycol) [mPEG]:  Proteomic Analysis of Plasma Protein Adsorption. Science China - Life Sciences, 55:191-201 (2012).  [NOTE:  Cover Art and featured article]

Kyluik-Price D.L., Li, L. and Scott, M.D.  Comparative Efficacy of Blood Cell Immunocamouflage by Membrane Grafting of Methoxypoly(Ethylene Glycol) and Polyethyloxazoline.  Biomaterials 35(1):412-422 (2014).

Li, L., Noumsi, G.T., Kwok, Y.Y.E., Moulds, J.M. and Scott, M.D.  Inhibition of Phagocytic Recognition of Anti-D Opsonized Rh D+ RBC By Polymer-Mediated Immunocamouflage.  American Journal of Hematology, 90(12):1165-1170 (2015).

Wang, D., Shanina , I., Toyofuku, W.M., Horwitz, M.S. and Scott, M.D.  Inhibition of Autoimmune Diabetes in NOD Mice by miRNA Therapy. PLoS ONE 10(12): e0145179. doi:10.1371/journal. pone.0145179 (2015).   Link:  Plosone Paper

Kyluik-Price D.L., Li, L. and Scott, M.D.  Effects of Methoxypoly (Ethylene Glycol) Mediated Immunocamouflage on Leukocyte Surface Marker Detection, Cell Conjugation, Activation and Alloproliferation.  Biomaterials 74(1):167-177 (2016).

Relevant Publications

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The 'immunocamouflage' of allogenic leukocytes with biocompatible polymers results in a dramatic shift in the immune status of the treated animal.  Shown are the differential effects of unmodied and mPEG-modidified allogenic splenocytes on T Regulatory (Treg) and proinflammatory Th17 (Teff)  lymphocytes in mice.  As shown, immune modulation arises consequent to the upregulation of regulatory T cells (Tregs) and the down regulation of effector (Teff; pro-inflammatory) T cells in the mice treated with mPEG-splenocytes.  The INCREASE in the Treg:Teff ratio induces a tolerogenic state in the treated animal.  In contrast, the unmodified leukocytes creates a proinflammatory state characterized by decreased Tregs and increased Teff cells.

Wang, D., Toyofuku, W.M., Chen, A.M. and Scott, M.D. Induction of Immunotolerance via mPEG Grafting to Allogeneic Leukocytes.  Biomaterials 32:9494-9503 (2011).

Kyluik-Price D.L., Li, L. and Scott, M.D.  Effects of Methoxypoly (Ethylene Glycol) Mediated Immunocamouflage on Leukocyte Surface Marker Detection, Cell Conjugation, Activation and Alloproliferation.  Biomaterials 74(1):167-177 (2016).

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