Scott Laboratory - Immios Biologics

Polymer-Based Bioengineering Solutions for Immune Modulation

Scott Laboratory Website

Canadian Blood Service • University of British Columbia • Centre for Blood Research • Vancouver, BC  Canada

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The 'immunocamouflage' of foreign cells and tissues via cell surface modification with biocompatible polymers such as methoxypoly(ethylene glycol) [mPEG] can be used to modulate the immune response both in vitro and in vivo.  This immune modulation arises consequent to the upregulation of regulatory T cells (Tregs) and the down regulation of effector (Teff; pro-inflammatory) T cells.  The INCREASE in the Treg:Teff ratio induces a tolerogenic state in the treated animal.

Polymer-Based Bioengineering for Immune Modulation

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The 'immunocamouflage' of allogenic leukocytes with biocompatible polymers results in a dramatic shift in the immune status of the treated animal.  Shown are the differential effects of unmodied and mPEG-modidified allogenic splenocytes on T Regulatory (Treg) and proinflammatory Th17 (Teff)  lymphocytes in mice.  As shown, immune modulation arises consequent to the upregulation of regulatory T cells (Tregs) and the down regulation of effector (Teff; pro-inflammatory) T cells in the mice treated with mPEG-splenocytes.  The INCREASE in the Treg:Teff ratio induces a tolerogenic state in the treated animal.  In contrast, the unmodified leukocytes creates a proinflammatory state characterized by decreased Tregs and increased Teff cells.

Wang, D., Toyofuku, W.M., Chen, A.M. and Scott, M.D. Induction of Immunotolerance via mPEG Grafting to Allogeneic Leukocytes.  Biomaterials 32:9494-9503 (2011).

Kyluik-Price D.L., Li, L. and Scott, M.D.  Effects of Methoxypoly (Ethylene Glycol) Mediated Immunocamouflage on Leukocyte Surface Marker Detection, Cell Conjugation, Activation and Alloproliferation.  Biomaterials 74(1):167-177 (2016).

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Kang, N., Toyofuku, W.M., Yang, X. and Scott, M.D. Inhibition of Allogeneic Cytotoxic T Cell (CD8+) Proliferation Via Polymer-Induced Treg (CD4+) Cells.  Acta Biomaterialia, 57:146–155 (2017).

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